NEXT day delivery available - 3pm cut off
Worldwide shipping available
Free standard UK delivery on orders over £100
This article is intended for qualified aesthetic practitioners. It is not patient advice and does not replace the manufacturer's instructions for use (IFU) or supervised training.
The standard Profhilo injection points are the five Bio Aesthetic Points (BAP) on each side of the face: the zygomatic protrusion, the nasal base, the tragus, the chin and the mandibular angle. Each point takes a 0.2 ml bolus, so a single 2 ml syringe covers all ten. The neck and the body have their own ten-point patterns, and Profhilo Structura is not a point technique at all, because it is delivered through a cannula. This guide sets out each pattern, the depths and volumes the published sources describe, and the errors that most often spoil an otherwise simple treatment.
Profhilo is an injectable medical device built from hybrid cooperative complexes of high- and low-molecular-weight hyaluronic acid, 32 mg of each in a 2 ml syringe. It is far more fluid than a cross-linked filler, so it is placed as a small bolus and left to spread, not threaded or sculpted. Writing in The PMFA Journal, Dalvi Humzah describes each bolus spreading to roughly 2 cm in diameter, which is why ten well-spaced deposits are enough for the cheek and lower face.
The fixed map also standardises the dose. Ten points at 0.2 ml use exactly one syringe, and the same pattern was used in the clinical evaluation of the product, so what you do in clinic matches what was studied. A map is not a substitute for anatomy, though. A published Profhilo BAP leaflet states that the points "must be adapted to the individual morphology of each patient".
The original open-label evaluation by Sparavigna and Tenconi (2016) treated 64 women aged 38 to 60 at five predetermined points per side, with a 0.2 ml bolus at each point through a 29G needle, in two sessions four weeks apart. The landmarks below combine that paper's description with the distances given in a published protocol summary.
Published distances differ by a few millimetres from one source to the next, so treat the current IFU and your training provider's map as the reference. Mark both sides with the patient sitting upright before you start, then step back and check symmetry. Soft tissue shifts when a patient reclines, so points marked lying down may not sit where you intended once they are upright.
Humzah's description is a 0.2 ml bolus placed deep dermally or subdermally, with the supplied 29G needle entering at 10 to 30 degrees. A small raised bleb at each site is expected, and gentle pressure afterwards helps it settle. Inject slowly, because a fast push through such a fine needle is more uncomfortable. A bolus placed too superficially stays visible for longer, so resist the temptation to go shallower in thin skin without a good reason.
The studied protocol is two sessions a month apart. Humzah notes that mature or sun-damaged skin may be given a third session a month later, with maintenance considered four to six months after the second treatment. Record the schedule you offer in the notes and explain it at consultation, so the patient understands that a single session is half a course.
The neck has its own ten-point BAP pattern, set out in an IBSA neck brochure. Again each point takes 0.2 ml. Four points sit on the midline: between the submental border and the hyoid, between the apex of the laryngeal prominence and the bottom of the thyroid cartilage, between the base of the thyroid cartilage and the sternal notch, and at the apex of the sternal notch. The remaining six are lateral, on horizontal lines at three levels. The highest is level with the mandibular angle and 0.5 cm lateral to the medial border of sternocleidomastoid. The lower two match the levels of the second and third midline points.
Depth matters more here than on the face. The brochure specifies the superficial subcutaneous layer and warns against approaching the skin at 90 degrees, which risks injecting deep to platysma. It also names the structures to stay clear of: the anterior and external jugular veins, the submandibular gland and thyroid, the carotid artery, and the trachea and larynx. Good lighting, a slightly extended neck and palpation of sternocleidomastoid before marking all make the lateral points easier to place. The brochure's schedule is two treatments a month apart, twice a year.
Profhilo Body comes as a 3 ml syringe containing 48 mg of high- and 48 mg of low-molecular-weight HA. A real-world evaluation of 50 patients published in 2024 describes ten intradermal injection points per area, arranged on three horizontal levels in a 3-4-3 pattern, with sessions on day 0 and day 30. The areas treated were the inner arms, abdomen, décolleté and knees. The kit is supplied with marking stencils, which take most of the guesswork out of spacing. Take the volume per point from the IFU, because body skin varies far more in thickness than facial skin.
Profhilo Structura contains 45 mg of each HA fraction in 2 ml and is intended for the superficial fat compartments of the lateral face, not the dermis. It is placed with a cannula, so the idea of "points" does not really apply. In the pilot study by Sparavigna and colleagues (2024), 50 women were treated through a single entry point 2 cm from the tragus with a 25G × 50 mm cannula and retrograde injection into the lateral cheek fat compartment, twice, one month apart. Light bruising at the injection sites was reported in 4 of the 50 and cleared within 5 to 10 days.
Harley Academy's summary of the training protocol describes two variants. For facial hollowing, a 22G × 50 mm cannula enters 2 cm from the tragus and delivers 1 ml per side into the preauricular superficial fat. For sagging, a 25G × 38 mm cannula enters 3 cm from the tragus, with 0.5 ml to the preauricular area and 0.5 ml along the zygomatic arch on each side. This is a different plane and a different instrument from classic BAP work, so it suits injectors who are already confident with cannulas. If you need spares in other gauges or lengths, the cannula range lists them by size.
Most problems come from drifting away from the map. Moving the zygomatic point medially towards the infraorbital area, or adding improvised points around the mouth, takes you outside the pattern that was studied, and any departure should be backed by specific training and the IFU. Splitting one syringe across face and neck leaves both areas under-dosed against the protocol.
The second group of errors is procedural. Skin should be disinfected and the injection carried out under aseptic conditions, as Humzah's account stresses. Screen every patient against the contraindications listed in the IFU, and defer treatment over inflamed or infected skin. Record the batch number, the points used, the volume at each and any deviation from the standard map, and take photographs at every visit. Those records are what you will rely on if a patient later reports a lump or asymmetry.
Finally, stock sensibly. Profhilo is supplied with 29G needles and Structura with its own cannulas, but check expiry dates and storage conditions on delivery for anything you order from the Profhilo range. If you are deciding whether bioremodelling or a different product class fits a patient, the wider skin booster collection is a useful way to compare formats.
On each side of the face: the zygomatic protrusion, the nasal base, in front of the tragus, the chin (marionette area) and the mandibular angle. Each receives 0.2 ml, giving ten points and 2 ml in total per session.
For the face, published descriptions place the bolus in the deep dermis or immediately subdermal plane with a 29G needle at a shallow angle of 10 to 30 degrees. For the neck, the IBSA brochure specifies the superficial subcutaneous layer and cautions against a perpendicular approach. Structura is different again and goes into superficial fat by cannula.
In the 2016 evaluation, "minor and temporary local skin reactions were observed in 23% of subjects", mainly light bruising and oedema at the injection points that cleared within 3 to 10 days. Visible blebs at the injection sites are expected at first. As with any injectable, infection, prolonged nodules and vascular events are possible, so practitioners should be trained and equipped to recognise and manage complications, and should work within their competence and insurance cover.
There is no guaranteed timeline. In the 2016 study, instrumental measures such as skin hydration and elasticity showed statistically significant change by weeks 4 to 8 and were still present at week 16, the final assessment. Individual response varies, and patients should be told so at consultation.
Profhilo injection points are simple to learn and easy to get slightly wrong. The face uses five BAP per side at 0.2 ml each, the neck uses ten points in the superficial subcutaneous layer with clear structures to avoid, the body uses a ten-point 3-4-3 pattern, and Structura replaces points with a cannula entry in front of the tragus. Mark carefully with the patient upright, respect the depth for each area, document what you did, and keep the current IFU as your final authority.
